Bibilical Illusions With The Novel Cry Essay Example for Free

Bibilical Illusions With The Novel Cry Essay Biblical reference within the story Cry the Beloved Country Many times in literary pieces, allusions are put in novels, used to foreshadow the ending of a book. The most common types of allusions are those from the bible. This is probably because many are familiar with the bible and its stories. The goal of foreshadowing is to provide a way for the reader to think more about the big picture, rather than what is happening page by page. In the novel, Cry the Beloved County, allusions to the Bible are very apparent and hold high significance in the story. The character Absalom shows tremendous similarities to the Biblical Absalom and almost seem to be made out of the same mold. Steven Kumalo from the novel and the simple man Job from the bible are tested in the same way and come out in the end in the same way. Biblical Absalom is essentially wronged by his half-brother Amnon, when his half brother forced his sister Tamar to sleep with him. Similarly Absalom Kumalo is wronged by his fathers, brothers, son, Matthew, when he will not admit to the court that he was an accessory to murder. Basically, both are wronged by a close family member, Biblical Absalom by his brother, and Absalom Kumalo by his cousin. In both stories, though not shown in depth, the men show a close trusting relationship with the one whom have wronged them. Before Amnon slept with his sister Tamar, Biblical Absalom would have no reason not to trust his brother, just as Absalom Kumalo would not doubt his cousin whom aided him in the murder. Later on, both men find out that, Amnon and Matthew are in it for themselves, not unlike both Absaloms. Absalom Kumalo shot Arthur Jarvis with the intentions of becoming richer. Even though Biblical Absalom killed his brother for sleeping with his sister, with Amnon dead, Absalom would inherit his fathers throne. (Bible p372-380) (Ward pp.188-189) In retrospect, both men were ultimately driven to commit the murder. Absalom Kumalo probably felt that if a white man can underhand him, and deny him money, then he deserves some of his fortune. So because whites men, kept blacks unskilled and did not allow them the same rights, many rebelled against the whites. In the same frame of mind, biblical Absaloms hatred for his brother was built up until he decided to go through with his murder. Even though Absalom Kumalo shot the white man on accident, he was doing it for his own gain. He went into the  house with the intention of stealing and instead shot a white man out of fear. Though it may seem that he regretted what he had done, which he in some ways did, his crime could not be fully repented. More than anything he probably wishes for Arthur Jarvis to be alive. He doesnt feels bad about killing him, he simply does not want to deal with the consequences. Biblical Absalom plans out the murder of his brother when he has him over for dinner. Neither men regret killing a man that was as family to them. (Bible p372-380) (Davis p184) Biblical Absalom murdered his brother Amnon for raping his sister Tamar two years earlier. Absalom Kumalo, on the same note, accidentally murdered a white man, while attempting to rob him. Absalom Kumalo, like biblical Absalom essentially murdered his own brother. Arthur Jarvis, the man Absalom Kumalo shot, was like a brother to the black men. He devoted his life to make a difference in blacks rights in South Africa. For both men, the act of murder on their part, ultimately led to their own demise. Biblical Absalom killed his brother Amnon and when king David found out that Absalom had indeed killed his brother Amnon. After the murder, both men flee the scene. Absalom Kumalo, runs because the price for killing a white man is high. Biblical Absalom flees because he also knows, that if he stays, hash consequences will be enforced. (Bible p372-380) Eventually, both man are captured and die in an unusual manner against both their fathers wishes. Absalom Kumalo is hung for his crime, so as to set an example. Though, the hanging of Absalom is not so strange in itself, its almost foretold in the biblical story of Absalom. After biblical Absalom flees his home on a horse, his head gets caught in a tree while passing under. This act is almost too familiar. As biblical Absalom dies with his body off the ground, struggling to get down, Absalom Kumalo dies with his head in a noose, and body dangling above the ground. (Bible p372-380) (Davis p184) In the bible, Job is developed as a man whom would never speak ill of god. Job is tested by Satan, who believes that Job will curse God if his prosperity is taken away. Satan kills Jobs children and all of his livestock, but Job does not lose his faith. Satan then takes away Job health by planting boils all over his body. Job eventually become very disappointed with his life and curses the day he was born. This is a classic example of how no man is perfect and when  Steven was introduced into Cry, the Beloved Country, he seemed to strive for perfection just as Job had in the bible. Job in theory seemed perfect but, Satan proved Job not to be, just as Stevens family proved him not to be. (Davis pp.265-272) (Bible p572-600) When brought into the story, both Steven and Job seem to be perfect and upright men. Steven, as a simple black parson and Job, as a upright man whom has shown no flaws. Though Stevens flaws are shown early on the novel, Cry, the Beloved County, he is still seen as a good man. Both men are examples of how bad things sometimes happen to good people. They both try to be the best men they can be, by fixing others mistakes and trying to be good people. Job offered burnt offerings to god everyday for his children who may have cursed god in their hearts and Steven prayed everyday to repent the sins of others and his own. The main thing that made these men eligible to lose so much, was because they had gained so much earlier in life. Though Steven want a rich man, he was far better off than any man in his village, and Job was a very prosperous man and looked well upon by god. (Davis pp.265-272) (Bible p572-600) Since Job was thought so highly of by God, he was tested by Satan in order to see if he would lose his faith. Steven Kumalo is tested in the same way as Job. Steven receives a letter from a priest informing him with the news that his sister, Gertrude, is sick. He leaves his normal life in the valley and travels to the city, with the intentions of bringing her home. When he arrives there, he is confronted with the horrifying news that she is selling herself to men. Steven goes in search of his son Absalom, and eventually finds him. Absalom had shot a white man and is sentence to be hung. Everything that went on in Jhonnaneburg hurt Steven and just like Job, he is tortured to the point where he will break if anything else were to happen. (Bible p572-600) (Ward p.222) In the end, though both men had lost practically everything they cared about, Steven his son and sister, and Job all his children and his prosperity, but then things got significantly better. Steven loses a son but gains a daughter carrying baby, and his sister Gertrudes son. Job loses all his children but gains ten new children and all his livestock back. Its an interesting coincidence how both men lose their child or children, but as they regain faith, new children are restored to them. (Davis pp.265-272)  (Bible p572-600) Paton used the name Absalom as an allusion in the story. His goal was probably to allow people to see into the future of Absaloms mortality. When I first read the story of Absalom, I didnt any definite similarities but as the stories start intertwining, I understood why he used the name Absalom for Stevens son. Absalom in general was a greedy boy, who really thought he deserved more and makes the mistake of killing a man which ultimately leads to his own death. Job and Steven, on the other hand, are generally men who strived to be good men. They were tested with their faith and both men failed, but they do gain their faith back, and their happiness along with it.

Culture Of Hepg2 Cells Biology Essay

Culture Of Hepg2 Cells Biology Essay Hep G2 cell line was purchased from American Type Culture Collection (ACTT) (VA, USA). Dulbeccos Modified Eagle Medium (DMEM), 0.5% Trypsin-EDTA 10x, and Penicillin-Streptomycin (PS) were obtained from Invitrogen Corporation (NY, USA). Fetal Bovine Serum (FBS) was gotten from Welgene Inc. (Daegu, South Korea). Fatty acids (Palmitic, Oleic and Dedocanoic acid), Dimethyl sulfoxide (DMSO) and Tween 20 came from Sigma (MO, USA). Bovine serum albumin (BSA) was from Santa Cruz Biotechnology (CA, USA). MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) was purchased from Molecular Probes (Oregon, USA). LDH assay (Lactate dehydrogenase assay) was from ROCHE (Mannhein, Germany). [emailprotected]/503 and Carboxyl-H2DCFDA were purchased from Invitrogen Corporation (Oregon, USA). Nile red was from Fluka (MO, USA). Triglyceride Quantification Kit and ATP Colorimetric/Flourometric Assay Kit were purchased from BioVion Inc. (CA, USA). Annexin V Floustaining kit was from Roche (IN, USA). Phosphate buffered saline was made up of chemicals at pH 7.4, including 11.7g NaCl, 5.5g Na2HPO4-7H2O, and 1.35g NaH2PO4. All other chemicals met in standard grade of analysis. Culture of HepG2 cells HepG2 cells were cultured in Dulbeccos modified Eagles medium, containing 10% (v/v) fetal bovine serum and 1% (v/v) Penicillin-Streptomycin under 5 % CO2, 95 % humidity at 37 °C. The cells were subcultured by using 0.5% Trypsin-EDTA 1x (Invitrogen Corporation, NY, USA) for detachment and seeded at proper cell number in all experiments. Fatty acid treatment When 80 % confluency of HepG2 was reached, it was treated with various concentrations of the fatty acids (0 mM, 0.1 mM, 0.2 mM, 0.3 mM, 0.5 mM, 0.7 mM and 1.0 mM) for 24 h. The stock solution of fatty acids was prepared at 100 mM by dissolving in DMSO and stored at -200C. The stocks were diluted in DMEM media containing a constant ratio of fatty acid bound bovine serum albumin at 2 to 1 to obtain working solution in all experiments. Cytotoxicity assay Cytotoxicity was based on the measurement of cytoplasmic enzyme activity by using cytotoxicity detection kit (ROCHE, Mannhein, Germany). The cytoplasmic enzyme was released from damaged cells that its enzyme activity expresses to the proportion of toxiced-cell. Lactate dehydrogenase (LDH) presents in all cells which is a stable cytoplasmic enzyme. When the membrane integrity of the cells is damaged, it is quickly released into the media. In this assay, NAD+ is reduced to NADH/H+ during conversion of lactate to pyruvate by the LDH-catalyzed. After that, H/H+ from NADH/H+ was transferred by the catalyst (diaphorase) to the tetrazolium salt (yellow) which was reduced to formazan (red). To conduct the assay, the culture supernatant is collected cell-free after desire exposure time (24 h). The reaction mixture from the kit was then applied in the samples. The absorption of the formazan dye formed was measured at 490 nm on an ELISA reader (VERSARMAX, Molecular Divices., CA, USA). Cell viability Cell viability was measured based on measurement absorption of a water-insoluble purple formazan which was reduced from a yellow water-soluble tetrazolium salt in live cells. Briefly, the cells were treated with MTT (5 mg/ml) in DMEM at 37 0C for 1.5 h. Then, the media were removed, and DMSO was added to dissolve the furmazan crystals. After gently pipetting, the absorbency was measured at 570 nm using an ELISA reader (VERSARMAX, Molecular Divices., CA, USA). The estimation of cell viability was calculated by comparing between the spectra value of treated and untreated cells. Quantification of triglyceride Triglyceride content (TG) was determined according to an enzymic method (BioVion Inc, CA, USA). In this method, glycerol is a product by TG-catalyzed which reacts with the probe to generate coloration measured on spectrophotometry at 570 nm. In briefly, the cells were washed twice times with cold PBS, then homogenized in 5% Triton-X100 solution. After slowly heating at 80-100 °C for 5 min, the samples were centrifuged at 12000 rpm for 5 min. The supernatant collected from removing insoluble materials was added 2 ÃŽ ¼l of lipase, mixed well and incubated for 20 min at room temperature. Finally, 50 ÃŽ ¼l of the reaction mix was putted in each sample for 45 min of incubation, protected from light. The value of triglyceride content was quantified based on triglyceride standard curve that was constructed with different concentrations of TG (0, 20, 40, 60, 80, and 100 nmol/ml). Measurement of reactive oxygen species (ROS) generation The measurement of ROS production within cells was carried out by using 2†²,7†²-Dichlorohydrofluorescein diacetate (Carboxyl-H2DCFDA; Invitrogen Corporation, Oregon, USA) which is combined into fluorescent products in the presence of H2O2 and other ROS molecules and esterases (Zhenyuan Song et al, 2007). After the cells were overloaded with 1.0 mM fatty acids, 10 mM final concentration of Carboxyl-H2DCFDA was added in the media without FBS at 370C in darkness for 30 min. Then, the cells were washed twice times with warmed PBS and lysed in 200 Ã‚ ­l RIPA buffer (PIERCE, IL, USA). The lysed-cells were centrifuged at 12000 rpm for 5 min. The supernatants were conveyed to a 96-well back plate which were excited at 485 nm and emitted at 530 nm for the Carboxyl-H2DCFDA fluorescence on Fluorometer (VICTOR2, Perkin Elmer., MA, USA). Trilyceride staining on Confocal Bodipy @493/503 (Invitrogen, Oregon, USA) was used to capture TG fluorescence on Confocal microscopy. In this experiment, the cells were prepared as above. Before the dyes treatment, the cells were washed with PBS twice times. Bodipy @493/503 was then added at 1.0  Ã‚ ­M, and 15 min of incubation at 370C after the cells were rinsed with PBS again. Zeiss LSM Image Brown software (LSM 510 meta, Carl Zeiss., Jena, Germany) was handled to take TG image at excitation of 488 and emission of BP 505-530 nm. ROS staining on Confocal ROS generation in HepG2 was stained by using Carboxyl-H2DCFDA. In this experiment, the cells were prepared as above. Before the dyes treatment, the cells were washed with warmed PBS twice times. The carboxyl-H2DCFDA was applied at 10 mM final concentration in Serum free media (DMEM without FBS), and incubated for 30 min at 370C, protected from the light. After that, the cells were rinsed with warmed PBS again. Zeiss LSM Image Brown software (LSM 510 meta, Carl Zeiss., Jena, Germany) was handled to take ROS image at excitation of 488 nm and emission of LP 530 nm. Detection of cell death and trilyceride accumulation by Confocal HepG2 seeded in the 24-well plate and treated with final concentration of fatty acids to 1.0 mM for 24 h. After the incubation time, the cells were washed twice times with PBS. Then, Bodipy @493/503 (Invitrogen, Oregon, USA) was dissolved in PBS at 5  Ã‚ ­g/ml which was added into each well. This process was kept in darkness for 15 min at 370C. After that, the Bodipy solution was removed and the cells were then washed by Binding buffer from Annexin V Floustaining kit (Roche, IN, USA). Finally, the cells were incubated in 100  Ã‚ ­l/ml of Propidium iodide (PI) for 10 min in darkness. Exposition of TG accumulation and apoptosis was observed at excitation of 488 and 543 nm, and emission of BP 505-530 and LP 650 nm on Carl Zeiss Confocal Microscopy (LSM 510 meta, Carl Zeiss., Jena, Germany), respectively. Data analysis All results were expressed as mean of repeated three or four values  ± SEM. The difference between groups was identified by using t.test. p < 0.05 was considered statistical significant.

Essay --

INTRODUCTION Advertising can mean many different things in today’s world. When advertising first was developed it was done by would of mouth and the classic flyer or poster, which is the traditional media. Then it moved up to using broadcast media such as radio to help capture a bigger audience. After that it moved towards the television where an even bigger audience could be reached. Lastly companies started to realize the shear amount of traffic that was generated by the Internet. The Internet first started to get popular in the mid 1990’s. Where only people with high tech computers and that could afford the service had the Internet. Of course the Internet did not look the way it does now during that time. There were no pop up ads, java, banners, or graphics that made a consumer purchase a product because they saw it on the Internet. One main reason that there was none of this was because the Internet could only use dial – up. Of course everyone knows how slowly that was, so picture trying to upload or update a website at that pace with huge files. We all know that this would take a very long time eventually making the company lose money. With the turn of the century close by not only did we enter a new century but we entered a new age of the Internet. The introduction of a cable modem drastically increased the Internet population. With speeds up to almost one hundred times faster then dial – up there was no comparison. With this new inven tion companies soon started to ease off on some parts of their advertising campaign and focused more on advertising on the Internet. So what is Internet Advertising? Internet Advertising is a way of marketing services or products on the World Wide Web. This can be done through search engine o... ... right people by increasing the awareness about the product, its benefits and drawbacks. This is important for the success of a business. There is both good and dark side of Internet advertising, including for social networking sites. As alluded to earlier, Internet marketing can manifest in negative, sometimes downright irritating, ways. Advertising malpractice can broach both the ethical and the legal. In e-mail marketing, knowing what constitutes spamming and what is legitimate advertising is extremely important. Internet threw open the plethora of opportunities for enormous scaling of business, thanks to the massive scope of expanding as well as popularizing the business by way of online advertisement. Now, every kind of business no matter big or small businesses can expand itself by way of online advertising where there are massive users across the whole world.

How to Use a Stethoscope Essay -- essays research papers

How to Use a Stethoscope Perhaps one of the greatest abilities in today’s medical world is the ability doctors and nurses have to listen to heartbeats, pulses, and breathing patterns with simplicity. It doesn’t require any high-tech equipment. It doesn’t require a myriad of tests and examinations. It doesn’t cost thousands of dollars per minute to operate. In fact, the abilities that I just mentioned are made possible by this instrument: a stethoscope. The stethoscope that we know today is not the stethoscope that was invented nearly 200 years ago. In 1816, a young physician in Paris, France, named R.T.H. Laennec, created the first recorded stethoscope, thanks to his noble convictions. You see, Dr. Laennec was examining a female patient, and was embarrassed to put his ear to her chest. This was common practice among physicians in this time period, but Laennec was simply resistant. Instead, he recalled that sound travels through solid materials. He rolled up 24 sheets of paper, and placed one end to his patient’s chest. The other end he placed to his ear, and to his amazement, listened to the noises of her chest cavity. Not only could he hear the sounds his patient was making, he noticed the sounds were louder and clearer.   Ã‚  Ã‚  Ã‚  Ã‚  Of course, Laennec’s design was much like a paper towel tube, so it is not at all representative of this instrument: the modern binaural stethoscope. This design was created only years after the creation of the stethoscope a...

Abortion: A Woman Has the Right to Choose Essay -- Argumentative Essay,

Abortion Introduction Abortion has become the common focus of diverse and influential debate in various societies, especially the U.S. One of the most confrontational topics argued is whether or not abortion is morally ethical. More than forty percent of all women will terminate their pregnancy by abortion at some point in their reproductive lives (Stacey OL). In spite of disagreement by many people, abortion is one of the most widespread medical procedures performed in the United States every single year. Eventhough abortion is opposed by many people, it should stay legal, because it is the right of a women to control herself or her body (Swomley, 1991). Pre-choice Abortion Abortion controversy can be broken down into two groups; those who oppose abortion and call themselves pro-life, and those who support abortion rights and consider themselves pro-choice. In 1916,the first family planning and birth control center in the United States was opened by Margaret Sanger. Fifty-seven years later in 1973, the Supreme Court of the U.S lined abortion legal during early stages of pregnancy to protect potential life in later stages, during case of Roe vs. Wade. Regardless of the court's ruling, abortion protests have grown from sidewalk picketing to clinic blockades, harassment of clinic workers and patients, vandalism, and even bombing, in the past thirty years. A fertilized ovum is less than the thickness of one human hair. It cannot be contemplated as the "baby". As thought upon by many people of humankind, it is outrageous to pretend that aborting the zygote or embryo is the same as "killing the baby", as pro-life movement emphasizes (Bettencourt, 1991). One of biggest arguments regarding the issue of abortion is whether or not l... ...hild later in life will decrease (Wattleton, 1991). Luckily, today in society, everyone is allowed to decide for themselves whether something is morally correct or incorrect. Ultimately, everyone is entitled to their own choices. Women should be permitted to resume choosing their personal preferences when it comes down to their bodies and reproductive lives. The advantages of abortion outweigh shortcomings. The case of Roe vs. Wade has assisted development of boundaries to conclude legal rights of the mother and the fetus. Although the pro-life and pro-choice group of persons are found, but there is nothing known as pro-abortion. While no one particularly wishes to end wonders of life, abortion guarantees protection and legal rights of women. Although abortion is opposed by many people, it should remain legal, as it is the woman's right to control her own body.

Machiavelli in Relation to Macbeth

Throughout history many have oversimplified and extorted Machiavellian idea s about power and adhering in an attempt to create empires and rule almost always at the expo ensue of the people they rule. Throughout the play Macbeth exhibits the ambition of a Machiavellian chart term, but often lacks the the form or any kind of rigidity that would land him firmly ammo nag Machiavellian clear cut classifications for power figures. Still it is clear based on the plot and details of Macbeth that Shakespeare was influenced by Machiavelli in writing his famous s â€Å"Scottish play†.As we read Macbeth we see the story of a man, Macbeth, filled with ambition who even begins the story in a mindset similar to that of a Machiavellian prince. In his most famous work Machiavelli cites a man named Stagecoaches â€Å"Stagecoaches, the Sicilian, became King of Syracuse, not only from a private position but from a low and abject one. This man, the son of a potter, through all the changes in his fortunes, always led an infamous life, nevertheless, he accompany ended his Wassermann 2 infamies with such great ability of mind and body, that, having devoting whims elf to the military profession, he rose through its ranks to be Praetor of Syracuse. (Mac heavily 4950) Macbeth is a ruler of what Machiavelli would have called a principality obtained through wickedness† (Machiavelli 49) Machiavelli belie Veda that â€Å"All states, all powers, that have held and hold rule over men have been an d are either republics or principalities. Principalities are either hereditary, in which the family has been long establish deed; or they are new. The new are either entirely new, as was Milan to Francesco Sports, or they are it were, members annexed to the hereditary state of the prince who has acacia red them, as was the kingdom of Naples to that of the King of Spain.Such dominions thus acquired are either accustomed to live under a prince, o to live in freedom; and are acquired eith er by the arms Of the prince himself, or of others, or else by fortune or by ability. † (Machiavelli 1) Under Machiavellian classifications, Macbeth is a grey area. His principality coo old potentially be considered hereditary because, although he had to murder Du NCAA he did technically inherit the throne, although, Macbeth is not a relative of Duncan s o the principality could be considered new. In her Tyranny in Shakespeare, Mary Ann McGill w rites that â€Å"[Macbeth] asks what is a tyrant?Or, what does a tyrant do that sets him apart from others Of great political ambition? † (McGill 21) Wassermann 3 When Machiavelli discusses â€Å"principalities obtained by arms† (Machiavelli 30) he states that â€Å"states which rise rapidly, like all other things that arise and grow rapidly in an true, cannot leave† This would lead one to believe that Macbeth is not Machiavellian in nature. Pee reaps not overall, yet Machete's reign of Scotland does still exhibit Machiavellian properties. For example where Machiavelli says: â€Å"Upon this a question arises: whether it be better to be loved than feared or feared than loved?It may be answered that one should wish to be both, but, because it is difficult to unite them in one person, it is much safer to be fear d than loved, when, of the two, either must be dispensed with. Because this is t o be asserted in general of men, that they are ungrateful, fickle, false, cowardly, covetous, and as long as you succeed they are yours entirely; they will offer y their blood, property, life, and children, as is said above, when the need is far distant; but when it approaches they turn against you.

Smallest Contribution Towards the Society

How can we contribute towards our society in the smallest possible way? We are apes,copycatsof west in Culture,Fashion,Independence,Freedom. We as individuals need to understand our responsibility. Our youths have loved their dresses,became party animals,night owls have engaged live-in relationships. Teenagers can be seen flaunting in front of their comrades. But friends i would like to raise a question ain't we trampling our freedom? Are we really behaving as human beings and not opportunist animals who encroach the shelters of others?In abroad no one makes parks,streets,pathways dirty by spitting,spilling left over drinksor beverages,chips wrappers,beverage cans etc. every Indian may be living in a small town,district,city,metro enjoys same menatility â€Å"keep your house clean† . If a person is living in a flat , they are not worried about the cleanliness of the space around stairs ,lift, outside their flat. If a person residing in a independent house they are not bothered about the street just following to their house ,opens stinking potholes.I want to cater attention of the so called civilised individuals busy discussing about politics ,technologies , duties , knowledge, when they themselves make the nation gardens ,streets deterrant by throwing away non-renewal plastic bags , banana peels disposable plats on the road. People blame goverment for not placing dustbins but what about places where scrap container are available but still we don't realize our duty and dispose garbage not in place. we go for picnics , parties ,long drives to enjoy nature ,food and return home after having a blast leaving that place in a mess.It leaves me flabbergasted that the destination point or the road leading to it can remain so beautiful if we are not selfish and think of others and make it a point that we are not going to dispose any waste in open surroundings. We are 1. 2 billion why engage more people in maintaining or cleaning but to emphasize that large chunk o f our population should be engaged in creation . We can have not only our homes clean but offices,markets,parks,colleges,,schools,temples,mosques,churches,railway stations,cinema halls/multiplexes,toilets etc.Only one thought of treating every public place as your own home and trying to keep that place clean for the next user can be real help for our society. A large amount of money to maintain public places can be saved if we show our concern and just dispose garbage at proper place allocated for it. Large amount of funds which are wasted in cleaning the clutter can be channelised and used for the upliftment of the under privileged,downtrodden. May lord convey this polite request of care and concern about others to all the citizens